The Dengue RNA Detection by Real-Time PCR – CSF (OS) test is a highly sensitive molecular method to find dengue virus genetic material (RNA) in Cerebrospinal Fluid (CSF), specifically useful for diagnosing neurological complications like dengue encephalitis or meningitis, confirming infection early (first week of illness), and identifying serotypes, using specialized PCR equipment to amplify and quantify viral DNA from spinal fluid samples